CENAS

Login

Dr. Devi B. Basnet

Research Director
Kathmandu

ACADEMIC QUALIFICATION

 

2005                           Ph. D. (Biochemistry) Sun moon University, South Korea

1997                           MSc.  (Chemistry) Tribhuvan University, Kathmandu, Nepal

1995                           BSc.   (Biology) Tribhuvan University, Kathmandu, Nepal

 

Research interests

  • Antibody and antigen related work
  • Streptomyces work
  • Bioinformatics

           

Work Experience

  1. Senior Researcher (2008-Present) Medytox, Inc. Ochang, South Korea

Project- Various protein display technologies (including phage and yeast display) including library design & generation, antibody selection, and clone characterization; humanization and affinity maturation of monoclonal antibody, stable cell line development and its characterization.

  1. Postdoctoral Fellow (2006-2008) Ewha Woman’s University, Seoul, South Korea

Project- Combinatorial biosynthesis of Ketolides in which the intermediates for its synthesis were biosynthesized by generatingdeletion/substituted mutants of Saccharopolyspora erythraea

  1. Lecturer (1998-2000) United Academy, Kathmandu Nepal; Little Angel’s College, Hattiban Kathmandu, Nepal; Sagarmatha College, Dillibazar, Kathmandu, Nepal

 

Publications

  1. Mo, SangJoon; Kim, Dong Hwan; Lee, Jong Hyun; Park, Je Won; Basnet, Devi; Ban, Yeon Hee; Yoo, Young Ji; Chen, Shu-Wei; Park, Sung Ryeol; Choi, Eun Ae; Kim, Eunji; Jin, Ying-Yu; Lee, Sung-Kwon; Park, Ju Yeol; Liu, Yuan; Lee, Mi Ok; Lee, Keum Soon; Kim, Sang Jun; Kim, Dooil; Park, Byoung Chul; Lee, Sang-gi; Kwon, Ho Jeong; Suh, Joo-Won; Moore, Bradley; Lim, Si-Kyu; Yoon, Yeo Joon (2011) Biosynthesis of the Allylmalonyl-CoA Extender Unit for the FK506 Polyketide Synthase (PKS) Proceeds Through a Dedicated PKS and Facilitates the Mutasynthesis of New Analogs, Am. Chem. Soc., 133 (4), pp 976–985.
  2. Basnet, D.B., Park, J.W. and Yoon, Y.J. (2008) Combinatorial biosynthesis of 5-O-desosaminyl erythronolide A as a potent precursor of ketolide antibiotics, J Biotech. 135 1, 92-96.
  3. Basnet, D.B., Oh,T.J.,Hang, T.T.V., Sthapit, B., Liou, K., Lee, H.C., Yoo,J.C., and Sohng J.K.(2006) Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: Cloning, Sequencing, and Characterization, Cells, 22, 2, 154-162.
  4. Simkhada, D.; Basnet, D.B, Lee, C.H., and Sohng, J.K. (2006) Cloning and expression of nucleotidyl transferase (espS1) gene from esperamycin producing strain Actinomadura verucososporaATCC J. Bio. Recont. vol. 3, No1, 31~38.
  5. Lee, S.K., Basnet, D.B., Hong, J. J., Jung, W.S., Choi, C.Y., Lee, H.C., Sohng, J. K., Ryu, K.G., Kim, D.J., Ahn, J.S., Kim, B.S., Oh, H.C., Sherman, D.H., Yoon, Y.J. (2005) Structural diversification of macrolactones by substrate flexible cytochrome P450 monooxygenases. Advanced Synthesis and Catalysis, 347, 1369-1378.
  6. Parajuli, N., Basnet, D.B., Chung, Y.S., Lee, H.C., Liou, K., and Sohng, J.K. (2005) Biochemical characterization of a Novel thermostable glucose-1-phosphate thymidylyltransferase from Thermus caldophilus: Probing the molecular basis from its unusual thermo stability. Enzyme and Microbial Technology, 37, 402-409.
  7. Basnet, D.B., Liou, K. and Sohng, J.K.(2005) Cloning of enediyne polyketide synthase gene clusters from Actinomadura verrucososporaATCC 39334 as esperamycin producer; JBR02,02, 53~58.
  8. Basnet, D.B., Maharjan, J., Lee, H.C., Liou, K. and Sohng, J.(2005) Expression, characterization and modeling of glucose-1-phosphate thymidylyltransferase from Streptomyces ahromogenes var rubradiris NRRL 3061; JBR, 1,1; 1-8.
  9. Basnet, D.B., Sthapit, B.S.,Lee, H.C., Liou, K., and Sohng, J.K. (2005) Swapping of domains in iterative type of PKS exemplifying with exchange of domain between NCS and AviM; 02, 01,29-34.
  10. Kharel, M. K., Basnet, D.B., Lee, H.C., Liou, K., Woo, J.S., Kim, B.G., and Sohng, J.K. (2004) Isolation and characterization of the tobramycin biosynthetic gene cluster from Streptomyces tenebrarius. FEMS Microbiol Lett 230, 185-90.
  11. Lee, S.K., Basnet, D.B., Choi, C., Sohng, J.K., Ahn, J.S., and Yoon, Y. J. (2004) the role of erythromycin C-12 hydroxylase, EryK, as a substitute for PikC hydroxylase in pikromycin biosynthesis. Bioorg Chem 32,549-59.
  12. Kharel, M. K., Basnet, D. B., Lee, H. C., Liou, K., Moon, Y. H., Kim, J. J., Woo J. S., and Sohng, J.K. (2004) Molecular cloning and characterization of a 2-deoxystreptamine biosynthetic gene cluster in gentamicin-producing Micromonospora echinospora ATCC15835 Cells 18, 71-8.
  13. Kharel, M.K., Subba, B., Basnet, D. B., Woo, J.S., Lee, H.C., Liou, K., and Sohng, J.K. (2004)A gene cluster for biosynthesis of kanamycin from Streptomyces kanamyceticus: comparison with gentamicin biosynthetic gene cluster. Arch Biochem Biophys 429, 204-14.
  14. Parajuli, N., Basnet, D.B., Kim, B.G., Lee, H.C., Sohng, J.K. and Liou, K. (2004) Diversity of Cytochrome P450 complement (CYPome) in Streptomyces peucetiusATCC Proceedings of Korean Society of Industrial Microbiology, Applied Chemistry. 8, 151-154.
  15. Parajuli, N., Basnet, D. B., Chan Lee, H., Sohng, J.K., and Liou, K. (2004) Genome analyses of Streptomyces peucetius ATCC 27952 for the identification and comparison of cytochrome P450 complement with other Streptomyces. Arch Biochem Biophys 425, 233-41.

 

error: Content is protected !!
Scroll to Top